Development of a Generator to Power ATP-Driven Molecular Motors

[摘要] Here, we report a maximum ATP synthesis rate of 193 nmol/min/mg for thermophilic F{sub 1}F{sub 0}. This rate is somewhat lower than the previously observed maximum rate of 500-700 nmol/min/mg (Pitard et al., 1996). However, ATP synthesis rates vary considerably with experimental conditions, and our observed rates compare favorably with the wide range of rates (40-700 nmol/min/mg) observed by these authors. Future research will focus on maximizing the ATP synthesis rate by adjusting environmental conditions, including the lipid and cholesterol composition of the proteoliposomes.

[发布日期] 2002-10-23 [发布机构] Cornell University, Ithaca, NY

[关键词] Biosynthesis;59 Basic Biological Sciences;Cholesterol;Atp;Lipids