Demonstrating biocompatibility with supercritical CO₂ : biphasic cultivation of Bacillus spp. and probing acclimation mechanisms through proteome and lipid analysis

[摘要] Supercritical (sc) CO₂ usage is increasing globally with applications as a sterilizing agent, as a non-toxic solvent, and as the form of the greenhouse gas CO₂ injected underground for geologic carbon sequestration (GCS). In this thesis I have described the isolation of microorganisms from three different carbon sequestration pilot sites through a novel method of successive scCO₂ enrichments. I show that microorganisms of the genus Bacillus, including GCS site isolates, are resistant to the bactericidal properties of scCO₂ , and can germinate and grow in an aqueous phase incubated under scCO₂ (Chapter 2). Bacterial resistance to scCO₂ challenges the efficacy of scCO₂ based sterilization and indicates that microbial activity may be harnessed in engineered environments containing scCO₂ (e.g. biochemical catalysis involving scCO₂ as a solvent or biofilm/biomineralized barriers to scCO₂ leakage from GCS sites). In an effort to understand the physiology of acclimation to scCO₂, I have sequenced and analyzed the genomes of two GCS-site isolates, B. cereus MIT0214 and B. subterraneus MITOTI (Chapter 3). I have used genome-enabled analysis of the proteome combined with analysis of membrane lipids to ask whether cellular macromolecules are differentially represented in cells grown under different headspace and pressure conditions including CO₂ and scCO₂ (Chapters 4). In this chapter I have examined the following three hypotheses regarding the mechanisms employed by Bacilli to resist scCO₂ : (1): Resistance to CO₂ stress is governed by a similar response as acclimation to low pH stress. (2): Cell wall and membrane alterations promote bacterial growth under scCO₂ by modulating the cell;;s microenvironment. (3): Global expression of proteins mediating cellular homeostasis in viable but non-growing (stationary-phase) populations acclimated to scCO₂ resembles a generalized profile of anaerobic growth, with notable exceptions of individual protein(s) that mediate acclimation. The results from this thesis enhance understanding of bacterial resistance to scCO₂, enabling improved strategies for scCO₂-based sterilization and accelerating biotechnological applications of scCO₂-biocompatible organisms.

[发布机构] Massachusetts Institute of Technology