Antioxidant and Antibacterial Studies of Leaves Extracts From Barkleyanthus salicifolius (Kunth) H. Rob & Brettel

[摘要] BackgroundBarkleyanthus salicifolius is a medicinal plant traditionally used to treat gastrointestinal conditions.ObjectiveThis study aimed to validate the popular ethnomedical use of Barkleyanthus salicifolius by evaluating its in vitro biological potential and characterizing its secondary metabolites.MethodsAntioxidant activity was determined by the DPPH radical inhibition assay, and antimicrobial properties were determined by microdilution to establish the Minimum Inhibitory Concentration (MIC). The phytochemical profile of the less polar lipophilic fractions was analyzed by gas chromatography-mass spectrometry (GC-MS).ResultsThe methanolic extract exhibited outstanding antioxidant capacity (87.58% DPPH inhibition at 100 ppm), comparable to quercetin, suggesting a high content of cytoprotective phenols. In the antimicrobial assays, the acetone extract showed remarkable activity against Staphylococcus aureus (MIC = 125 µg/mL). Furthermore, in conjunction with the hexane extract, it inhibited MRSA strains (MIC = 250 µg/mL). An inhibitory effect against Pseudomonas aeruginosa was also observed. GC-MS analysis revealed the presence of austricin, epiglobulol, and α-cadinol in the lipophilic fractions.ConclusionThe findings position Barkleyanthus salicifolius as a promising source of antioxidant and antibacterial agents. These results scientifically support its traditional ethnomedical use through the identification of its main bioactive compounds.

[发布日期] 2026-07-01

[关键词] antioxidant activity;antibacterial activity;medicinal plant;minimum inhibitory concentration;bacterial resistance