HPMA-oligolysine copolymers for gene delivery: Optimization of peptide length and polymer molecular weight

[摘要] Polycations are one of the most frequently used classes of materials for non-viral gene transfer in vivo. Several studies have demonstrated a sensitive relationship between polymer structure and delivery activity. In this work, we used reverse addition-fragmentation chain transfer (RAFT) polymerization to build a panel of N-(2-hydroxypropyl)methacrylamide (HPMA)-oligolysine copolymers with varying peptide length and polymer molecular weight. The panel was screened for optimal DNA-binding, colloidal stability in salt, high transfection efficiency, and low cytotoxicity. Increasing polyplex stability in PBS correlated with increasing polymer molecular weight and decreasing peptide length. Copolymers containing K-5 and K-10 oligocations transfected cultured cells with significantly higher efficiencies than copolymers of K-15. Four HPMA-oligolysine copolymers were identified that met the desired criteria. Polyplexes formed with these copolymers demonstrated both salt stability and transfection efficiencies on-par with poly(ethylenimine) PEI in cultured cells. (C) 2011 Elsevier B.V. All rights reserved.

[发布日期] 2011-10-30

[效力级别]  Proceedings Paper

[关键词] Non-viral gene delivery;Polyplex;Peptide copolymer;HPMA;RAFT polymerization