ALLERGENS IN BEE VENOM .2. 2 NEW HIGH MOLECULAR-WEIGHT ALLERGENIC SPECIFICITIES

[摘要] Two new allergenic specificities were detected in honeybee venom and the 2 corresponding protein substances isolated by gel filtration, immunoadsorption and ion exchange chromatography. The 1st of these, allergen B, has a MW ranging from 49,000 to more than 200,000 d [daltons] and can be recognized by rabbit and guinea pig antisera and by human reaginic sera using the radioallergosorbent test (RAST). Allergen B gives a single line in immunodiffusion distinct from hyaluronidase, phospholipase A, melittin and the other high MW substances described and gives a single band at 49,000 d in sodium dodecyl sulfate (SDS) polyacrylamide gel. The 2nd substance, allergen C, has a MW of 105,000 d and was separated from allergen B by immunoadsorption with insoluble antibody. Allergen C was distinct from the other substances in bee venom by immunodiffusion with animal antisera. One human reaginic serum was monospecific for allergen C. Two other minor components of 86,000 and 71,000 d are present in bee venom; their allergenic activities are unknown. The 2 specificities, B and C, comprise most of the reactivity of the previously described Sephadex G-75 fraction 1 and clearly are important allergens, reacting with 98% of sera from bee venom-allergic individuals.