PASSIVE TRANSFER OF EXPERIMENTAL HYPERSENSITIVITY PNEUMONITIS WITH LYMPHOID-CELLS IN RABBIT

[摘要] Although precipitating antibody is associated with human hypersensitivity pneumonitis, cell-mediated hypersensitivity may be involved in disease pathogenesis. In this study, alveolar, interstitial and peribronchial lesions were produced by respiratory challenge of rabbits passively sensitized with ovalbumin-sensitive lymph node cells. Ovalbumin sensitivity of donor rabbits and lymph node cells was demonstrated by skin testing, migration inhibition factor (MIF) production using alveolar wash cells as migrating cells, and lymphocyte stimulation. Passive cell transfer was accomplished by i.p. injection with all lymph node cells obtained from 1 donor transferred to 1 recipient. Recipients were challenged by aerosol or intratracheal injection of antigen immediately or 24 h after passive sensitization and were killed 48 h after challenge. Lesions in rabbits passively sensitized by lymph node cells and challenged with antigen by intratracheal inoculation consisted of focal pneumonitis with intra-alveolar edema and infiltrates of mononuclear cells in alveoli and alveoli septa. Aerosol challenge of passively sensitized animals produced similar changes, but peribronchial lymphoid tissue containing macrophages and germinal centers was prominent in this group. Antiovalbumin serum recipients challenged by intratracheal injection demonstrated only mild peribronchial mononuclear cell infiltrates, without pneumonitis. Control animals receiving lymph node cells only or challenge only demonstrated no changes in lung histology.