ALLERGENS OF MAMMALIAN ORIGIN .4. EVIDENCE FOR COMMON ALLERGENS IN CAT AND DOG SERUM

[摘要] IgE [immunoglobulin E] antibodies present in serum from 3 patients clinically sensitive to cat and dog combined with whole cat and dog sera linked to cellulose particles. Employing a modified radioallergosorbent technique (RAST), it was shown that cat and dog sera inhibited the binding of IgE antibodies to insolubilized cat serum and to insolubilized dog serum. Cat and dog sera apparently have common allergens. Other mammalian sera [horse, rabbit, rat, mouse, guinea pig] were also tested and had little inhibitory activity. Cat and dog sera did not inhibit the binding of IgE anti-ragweed antibodies to an insolubilized ragweed fraction. Following separation of cat serum by gel filtration on Sephadex G-200 or electrophoresis in acrylamide gel, several fractions inhibited the binding of IgE antibodies to insolubilized cat and dog sera. It is not known if the heterogeneity observed reflects the presence of single allergens with diverse size and charge properties or multiple allergens.