PROPERTIES OF MOUSE HOMOCYTOTROPIC AND HETEROCYTOTROPIC ANTIBODIES

[摘要] Mouse antisera were analyzed for the presence of homocytotropic and heterocytotropic antibodies. Two distinct populations of antibodies were detected by the homologous passive cutaneous anaphylaxis (PCA) reaction. The 1st was active 2 h after injection, heat-stable and partially reactive with [goat] antisera to mouse IgG1 [immunoglobulin G1]. The 2nd was active 48 h after injection, was heat-labile and probably belonged to the IgE class. There were 2 antibodies active in the heterologous PCA reaction in rats, a heat-stable antibody and a heat-labile antibody. Contrary to homocytotropic antibodies, none of the heterocytotropic antibodies detected reacted with antisera to mouse IgG1 or IgG2. In addition to IgE, there may exist another heterocytotropic antibody in mouse antisera, and caution should be employed when using the 2-h PCA reaction in the rat as a sole criterion for detection of mouse IgE.