Early expression of Iε, CD23 (FcεRII), IL-4Rα, and IgE in the human fetus

[摘要] Background: A major predictor of childhood atopy is the concentration of IgE in the cord blood, but whether the source of cord blood IgE is maternal or fetal remains unclear. Objective: We sought to determine the pattern of in situ IgE production during ontogeny. Methods: Ninety-seven fetal, 142 natal, and 96 childhood samples were analyzed by using reverse transcription PCR for transcription of VDJC epsilon, I epsilon, and CD23. Thirty-eight fetal liver samples were analyzed for tile IL4RA genotype. Results: IL-4R alpha, CD23a, CD23b, and sterile I epsilon transcripts were present as early as 8 weeks' gestation. VDJC epsilon transcripts were found in second-trimester fetal liver and third-trimester cord blood, although they were rare. VDJCe transcripts were more common in the blood of children 9 months and older. Sequence analysis suggested that fetal VDJC epsilon was the product of selection. All fetal livers actively transcribing I epsilon, VDJC epsilon, and IL-4R alpha contained at least one copy of the atopy-associated IL4RA*A1902G polymorphism. Conclusion: The human fetus contains B cells that are primed to undergo IgE class switching from the earliest stages of ontogeny and can produce endogenous IgE by 20 weeks' gestation. However, IgE-producing cells are rare until 9 months after birth.

[发布日期] 2000-11-01

[关键词] IgE;immunoglobulin variable region;CD23;IL-4 receptor;receptors Fc;spleen;liver;cord blood;gut;mesentery;fetus;human