COMPLEMENT-MEDIATED RELEASE OF HISTAMINE FROM HUMAN BASOPHILS .3. POSSIBLE REGULATORY ROLE OF MICROTUBULES AND MICROFILAMENTS

[摘要] The release of histamine by normal human leukocytes (basophils) following in vitro challenge with activated complement (zymosan-treated serum) was previously reported. In this study, the effects of various pharmacologic agents on this release mechanism was compared with allergen-induced release of histamine. Colchicine and vinblastine antagonize the polymerization of tubulin to form microtubules, and both agents inhibited complement- and allergen-mediated release of histamine. The dose-response effects of these drugs were quite similar for the 2 release mechanisms. Deuterium oxide, an agent which stabilizes microtubules, enhanced complement- and allergen-triggered release of histamine from basophils. Treatment with cytochalasin B, a fungal product known to interfere with microfilament formation, resulted in enhanced release of histamine from complement-treated basophils but no significant change in the percentage of histamine released from allergen-treated basophils. Microtubules and/or microfilaments are apparently involved in complement-induced secretion of histamine by human basophils.