SUPPRESSION OF CONTACT SENSITIVITY BY IGG1 ANTI-HAPTEN ANTIBODY IN CONTACT-SENSITIZED GUINEA-PIGS

[摘要] The guinea pig sera obtained 2 wk after 1-fluoro-2,4-dinitrobenzene sensitization suppressed elicitation of contact sensitivity when transferred i.v. into contact sensitized guinea pigs. The activity was found only in the sera obtained 2 wk after the sensitization, whereas the sera obtained 1 and 3 wk after the sensitization had no effect on contact sensitivity. Pretreatment with cyclophosphamide abolished the generation of the suppressive factor. The suppressive factor was hapten-specific. It was eluted from a Sephadex G-150 column into 7S .gamma.-globulin fraction and from DEAE ion exchange cellulose column into IgG1 fraction. The suppressive activity was absorbed on a dinitrophenylated bovine gamma globulin Sepharose 4B column and was eluted by either acidic buffered solution by dinitrophenol from the affinity column. It was absorbed by antiguinea pig IgG1-Sepharose and not by antiguinea pig IgG2-Sepharose. The suppressive activity found in the sera of 1-fluoro-2,4-dinitrobenzene sensitized guinea pigs was attributed to IgG1 antihapten antibody.