BIOPHYSICAL CHARACTERIZATION OF NORMAL LYMPHOCYTES-T AND SEZARY CELLS

[摘要] Blood lymphocytes from 18 patients with cutaneous T-cell lymphoma (Sezary syndrome and mycosis fungoides) were characterized using multiparameter laser flow microfluorimetry (FMF) and automated image analysis (AIA). The results were correlated with routine blood smears, cytogenetic studies and observations made on PHA[phytohemagglutinin]-stimulated normal T-lymphocytes in vitro. Specimens from all 9 patients with Sezary syndrome and 5 of 9 patients with mycosis fungoides contained 1 or more discrete subpopulations of neoplastic (Sezary) lymphocytes that were detected by FMF. Studies with AIA demonstrated that neoplastic T lymphocytes are distinguished from normal quiescent (GO) lymphocytes by alterations in DNA content (aneuploidy) and by chromatin structuring (increased chromatin dispersion), which may be a more sensitive index of neoplastic transformation than ploidy levels. In several patients, small and large Sezary cells were present with DNA-chromatin properties quite similar to normal cycling G1 and G2 lymphocytes, respectively, but their presence was not explained by an increase in proliferative activity in the blood. Sezary syndrome apparently consists of a heterogeneous group of related disorders differing in terms of the Sezary cell population. The response to treatment and prognosis may differ accordingly.