LACTATE DEHYDROGENASE ISOZYME PATTERNS IN BLISTER FLUIDS

[摘要] The LDH isozymes patterns were determined in fluids from various bullous dermatoses as well as from liquid nitrogen induced blisters. Most specimens showed all 5 isozymes. A consistent finding was the marked preponderance of LDH 5 over all other isozymes and this was noted in all blister fluids regardless of their etiology or histologic structure (subcorneal, intra or subepidermal). Since LDH 5 is the major isozyme of the epidermis, this strongly suggests an active participation of epidermal cells in the formation of blister fluid. LDH isozyme patterns may change very drastically within short periods of time as the blister ages. For this reason it is difficult to assign any diagnostic specificity to the LDH isozyme patterns seen in blister fluids from various dermatoses.