PHASE-CONTRAST TIME-LAPSE CINEMATOGRAPHY OF CULTIVATED NORMAL AND PSORIATIC ADULT HUMAN SKIN

[摘要] Viable, sheet-like epithelial outgrowth and proliferation, apparently free of dermal elements, but attached to the split thickness skin explant from which the outgrowth originated, was produced from normal, uninvolved and involved psoriatic epidermis. Uninvolved, psoriatic and normal skin produced outgrowth not significantly different in the semi-quantitative method. A significant difference here was a factor 2 or 100%, which is decidedly smaller than the increased proliferation of psoriatic epidermis in vivo. Psoriatic skin produced outgrowth less frequently and less extensively. The outgrowth from psoriatic skin did not differ from that from uninvolved psoriatic or normal skin in growth pattern, epithelial morphology, mitoses, or by time-lapse cinematography observation. Increased dermal thickness of the explant, addition of autologous, whole red cells and whole white cells, and incubation at lower temperatures did not produce accelerated proliferation of epidermal cells from psoriatics. Disrupted, autologous red cells and white cells decreased epithelial outgrowth. Autologous serum from psoriatics caused peculiar epithelial cell alterations in several outgrowths from uninvolved skin of psoriatics. Also, some evidence is presented concerning a factor in psoriatic serum that increased and decreased epithelial outgrowth and a factor in normal serum that decreased epithelial outgrowth in the experimental system. Hep-arinization and heat inactivation did not influence the serum factor.