SEBACEOUS GLAND DIFFERENTIATION .3. THE USES AND LIMITATIONS OF FRESHLY ISOLATED MOUSE PREPUTIAL GLAND-CELLS FOR THE INVITRO STUDY OF HORMONE AND DRUG-ACTION

[摘要] The effects of selected hormones and drugs on freshly isolated mouse preputial gland cells were studied. Testosterone, DHT [dihydrotestosterone], androstanediol, androsterone and androstanedione failed to stimulate and estradiol failed to inhibit DNA or lipid synthesis. Thyroxine and insulin had no effect on lipogenesis but epinephrine and PGE2 [prostaglandin E2] caused significant stimulation as did Bt2cAMP [dibutyryl cAMP]. The antilipemic drugs clofibrate, nicotinic acid and hydroxycitrate inhibited lipogenesis. Of anti-acne drugs, only L-dopa inhibited lipogenesis, tetracycline or trans-retinoic acid showed no effect. Pyridoxine was unable to inhibit lipogenesis but DMSO [dimethyl sulfoxide] caused dramatic stimulation, although it was without effect on DNA synthesis. Evidence was presented which suggested that the lack of response to steroid hormones was not due to the inability of the cells to take up and metabolize the steroids but that the time-span of exposure was not long enough to elicit a cellular response. Freshly isolated cells were suitable for the study of those effects of hormones and drugs which occur within the 1st 3 h after exposure to the compound.