IN VITRO CULTURE OF HUMAN SKIN EPITHELIAL CELLS

[摘要] The origin and identification of cells from primary cultures of post-embryonic human skin were determined, and the physical and chemical parameters that affect growth were studied. Epithelial outgrowth occurs by a process of independent cell multiplication as shown by radio-autography with tritiated thymidine. Increased O2 tension, serum concentration, and pH either completely inhibit or markedly retard epithelial cell growth. Electron micrographs of epithelial cells in culture reveal morphologic detail (tonofilaments, desmosomes) characteristic of epithelial cells observed in vivo. Protoplasmic filaments which connect migrating epithelial cells are described.