IDIOPATHIC HYPERLIPEMIA AND PRIMARY HYPERCHOLESTEREMIC XANTHOMATOSIS .2. ANALYSIS OF THE PLASMA PROTEINS AND LIPIDS BY MEANS OF ELECTROPHORESIS AND FRACTIONATION OF THE PLASMA PROTEINS - EFFECT OF HIGH SPEED CENTRIFUGATION AND OF EXTRACTION WITH ETHER ON THE PLASMA PROTEINS AND LIPIDS

[摘要] Electrophoretic analysis showed that in idiopathic hyperlipemia either the alpha-2 peak alone or both the alpha-2 and beta-1 peaks are elevated. In primary hypercholesteremic xanthomatosis beta-1 peak is always increased. Elevation of the peaks in both diseases was caused by lipid substances. Fractionation of the plasma proteins revealed in both diseases a great increase in beta-1 lipoprotein. Values for alpha-1 lipoprotein were generally normal. The alpha-1 and alpha-2 glycoproteins were present in normal amounts, whereas values for beta glyco- proteins were moderately elevated in both diseases. High speed centrifugation of the clear plasma of patients with primary hypercholesteremic xanthomatosis induced no changes in the electro-phoretic pattern or in amounts of cholesterol, phospholipids, neutral fat and N in the plasma. In idiopathic hyperlipemia it resulted in partial or complete clearing of plasma with accumulation of a creamy layer of lipid material on top, a decrease in size of previously elevated alpha-2 and beta-1 peaks in the electrophoretic pattern, and a considerable decrease of neutral fat, cholesterol and phospholipids but not of N in the plasma. In patients with primary hypercholesteremic xanthomatosis and idiopathic hyperlipemia, just as in normal individuals, all lipids in the plasma seem to be bound to proteins in some way. This binding holds the lipids in suspension or solution. The neutral fat is present largely as chylomicra while the cholesterol and phospholipids are present largely as lipoproteins.