ISOCITRIC DEHYDROGENASE ACTIVITY OF HUMAN EPIDERMIS

[摘要] An extremely sensitive and reproducible method is described for measuring the isocitric dehydrogenase activity of skin. The method has been used for homogenates of human epidermis and for fragments of skin appendages weighing 1.0 to 5.0 [mu]g dry weight. Epidermal homogenates have a mean activity of about 0.24 [image]/kg wet weight/hour. Analysis of frozen dried skin sections show that the mean activity of epidermis, dermis, hair follicle, sebaceous gland and sweat glands are respectively 1.25, 0.08, 0.87, 2.84, 1.86 [image]/kg dry wt/hour. These findings are discussed in relation to the carbohydrate mechanism of skin, and in conjunction with other evidence suggest that the tricarboxylic cycle functions in skin.