QUANTITATIVE HISTOCHEMISTRY OF PRIMATE SKIN .4. ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE

[摘要] [alpha]-Glycero-phosphate dehydrogenase activity in the [rhesus and stump-tail macaque] skin and appendages is generally much lower than other enzymes participating in glycolysis. The epidermis has approximately 100 mmoles/hr./kg dry wt. of activity, which is 1/5 that of hexokinase or fructoaldolase activity and 1/50 that of glyceraldehyde-3-phosphate dehydrogenase activity of the same epidermis. It is possible that the main physiological role of this enzyme in the epidermis and mucous membrane may be concerned with the synthesis of glycerophosphatides, and not with anaerobic glycolysis. This enzyme''s activity is relatively high in sebaceous and sweat glands, i.e. 850 to 1100 mmoles/hr./kg dry wt. The values are of the same order of magnitude as compared with hexokinase or fructoaldolase activities, and 1/4-1/6 those of glyceraldehyde-3-phosphate dehydrogenase activities in glandular tissues. In sebaceous and sweat glands, this enzyme may participate in glycerophosphatides synthesis as well as in the anaerobic glycolytic pathway.