MUTAGENICITY OF URINE FROM PSORIATIC PATIENTS UNDERGOING TREATMENT WITH COAL-TAR AND ULTRAVIOLET-LIGHT

[摘要] The possible percutaneous absorption of mutagens from patients receiving crude coal tar (CCT) and UV light was investigated. Urine samples were collected from nonsmoking volunteers, smoking and non-smoking psoriatic patients. Patients were treated with 1% CCT USP or 1-10% CCT in petrolatum in the evening. The following morning, patients received coal tar baths and then UV light (mainly 290-320 nm, UVB [shortwave UV light]). Nonpolar organics in urine samples were extracted by adsorption onto XAD-2 resin and the extracted organics assayed in the Ames Salmonella/microsome test. TA98 was the most sensitive bacterial strain to detect mutagenicity. Except for smoking psoriatic patients, the addition of liver homogenate was necessary to see mutagenicity. No increase in the number of revertants was observed when .beta.-glucuronidase was added to the assay. Of 14 patients studied, 12 had at least 1 mutagenic urine sample. Typical values for nonsmoking psoriatics treated with CCT were 42-496 his+/20 ml of urine after the subtraction of spontaneous his+ counts (26 .+-. 6). Two nonsmoking normal volunteers excreted mutagenic urines; for smoking psoriatic patients, 213-1100 his+/20 ml urine. The percutaneous absorption of mutagens from CCT was demonstrated; its effects may not be limited to the skin.