COMPARATIVE HISTOCHEMICAL OBSERVATIONS ON WOUND HEALING IN ADULT RATS AND CULTURED ADULT HUMAN EPITHELIUM .1. METHODS AND GLYCOGEN DISTRIBUTION

[摘要] Glycogen distribution in the epidermal cells during wound healing of 32 rat burns was compared with its distribution in 210 explants of cultured human skin. A technique of culturing on collodion membranes was used in order to embed and section the explants without disturbance of normal relationships to the outwandering cells. Normal rat skin contains little or no histochemically detectable glycogen. During wound healing all layers of the migrating epithelium except the basal layer contain large quantities of glycogen. This persists until keratinization of the epi-thelialized wound occurs at which time the glycogen disappears. Normal human skin contains only slight quantities of glycogen, but the out wander -ing cells during culture accumulate large stores of this substance. Unlike the distribution of glycogen in burns it also appears in the cells of the inferiormost layer of the outgrowth during culture. Whole mounts of the cultures reveal the location of glycogen to be in the perinuclear cytoplasm immediately following mitosis. In the resting cell it is diffusely scattered throughout the cytoplasm. This parallelism suggests that cultured material undergoes the same carbohydrate metabolic processes as those occurring in wound healing.