THE HISTOCHEMICAL RESPONSE OF THE HUMAN EPIDERMIS TO CONTROLLED INJURY - GLYCOGEN

[摘要] The epidermis of normal adult young men was injured by the Strip Method of Pinkus. Biopsies were taken at various intervals after injury and stained with the McManus technique for glycogen and other Schiff-positive materials. Two major accumulations of glycogen were observed in the epidermis: (a) in the basal cells of the epidermis at 8, 16, and 24 hrs. after the completion of injury; (b) in the spinosum cells of the mid-epidermis at 3 to 5 days after injury. Of equal interest and perhaps significance is the total absence of glycogen and all Schiff-positive matter, (a) in the swollen basal cells immediately after injury, (b) in all epidermal cells 4 hrs. after injury, (c) in the outer zone of epidermis that includes the horizontally arranged and flattened spinosum cells and the granular cells (designated as Zone 3) throughout the entire expt. These histochemical changes were correlated with the cellular activity of the epidermis and discussed accordingly.