QUANTITATIVE HISTOCHEMISTRY OF PRIMATE SKIN .V. GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND 6-PHOSPHOGLUCONATE DEHYDROGENASE

[摘要] The distribution of glucose-6-phosphate and 6-phosphogluconate dehydrogenases in the skin of rhesus and stump-tail macaques was analyzed quantitatively with a fluorometric method. Although the specific activity of G-6-PDH was approximately twice as high as that of 6-PGDH, the distribution of both dehydrogenases is similar in the cutaneous organs. High enzyme activities (1.0-1.9 moles/hr. /kg dry wt. for G-6-PDH and 0.4-6.2 moles/hr./kg dry wt. for 6-PGDH) were found in the sebaceous glands, hair follicles and apocrine sweat glands. Lower activities (0.25-0.57 moles/hr./kg dry wt. for G-6-PDH and 0.13-0.35 moles/hr./kg dry wt. for 6-PGDH) were observed in the eccrine sweat glands except for those in the bald scalp of the stump-tail macaque. The NADP-linked dehydrogenases studied here were concentrated more in the upper layers than in the basal layer in the epidermis of the sole. The quantitative distribution patterns of these 2 enzymes suggest an active participation of the pentose phosphate shunt of glucose metabolism in various cutaneous organs.