IDIOPATHIC HYPERLIPEMIA AND PRIMARY HYPERCHOLESTEREMIC XANTHOMATOSIS .6. STUDIES OF THE SERUM PROTEINS AND LIPOPROTEINS BY MOVING BOUNDARY ELECTROPHORESIS AND PAPER ELECTROPHORESIS BEFORE AND AFTER ADMINISTRATION OF HEPARIN

[摘要] In idiopathic hyperlipemia moving boundary electrophoresis showed that the chylomicra which largely cause the turbidity of the serum have different electrophoretic mobilities corresponding to those of beta-1 globulin, alpha-2 globulin, alpha-1 globulin and albumin. Moving boundary electrophoresis in idiopathic hyperlipemia, after an injection of heparin, showed a decrease of the alpha-2 and beta-1 peaks to subnormal values and the appearance of a pre-albumin component. Paper electrophoresis showed that these changes were due to an increase in the electrophoretic mobility of both the alpha and beta lipoproteins and that the prealbumin component represented the alpha lipoproteins. The same electrophoretic changes occurring after an injection of heparin as seen in idiopathic hyperlipemia were observed in normal persons with alimentary hyperlipemia and in patients with hyperlipemia due to nephrosis. Moving boundary electrophoresis in primary hypercholesteremic xanthomatosis, after an injection of heparin, showed in 2 of 4 patients no changes from the pre-heparin pattern and in 2 patients a decrease of the beta-1 globulin peak and an increase of the alpha-2 globulin peak. Paper electrophoresis showed that these changes were due to an increase in the electrophoretic mobility of the beta lipoproteins; whereas the alpha lipoproteins showed no changes in their mobility. The electrophoretic changes produced by a single intravenous injection of heparin persisted much longer in idiopathic hyperlipemia than in primary hypercholesteremic xanthomatosis; about 16 hours in the former, and less than 1 hour in the latter. No electrophoretic changes after an injection of heparin were observed in strictly fasting normal persons.