MECHANISM OF ACANTHOLYSIS - THE EFFECT OF CANTHARIDIN ON OXIDATIVE PHOSPHORYLATION

[摘要] Disodium cantharidin (DSC), a compound which produces acantholysis in the epidermis, inhibited oxidative phosphorylation in rat liver mitochondria. The data indicate that the site of inhibition is in the sequence of phosphorylating reactions. Aged preparations of mitochondria showed a marked uncoupling of phosphorylation from oxidation. Methylene blue and 2,4-dinitrophenol completely or partially reversed the inhibition of oxygen uptake by DSC. These compounds also prevented cantharidin-induced acantholysis in vitro. Acantholysis requires energy which is supplied by oxidative phosphorylation.