DNA DAMAGE IN HUMAN-SKIN FIBROBLASTS EXPOSED TO UVA LIGHT USED IN CLINICAL PUVA TREATMENT

[摘要] Human skin fibroblasts were irradiated with a clinically used UVA light source. The doses (1.1 and 3 J/cm2) were similar to those reaching the dermis during clinical PUVA [psoralen plus UVA] treatment of psoriasis. DNA strand breaks, as determined by alkaline elution, were formed in a dose-dependent way and disappeared within 1 h of postincubation at 37.degree. C. These findings have clinical implications since UVA-induced DNA damage may be accompanied by mutagenic and tumor promoting effects.