SKIN CALCIUM-BINDING PROTEIN IS LOCALIZED IN THE CYTOPLASM OF THE BASAL LAYER OF THE EPIDERMIS

[摘要] Using a serum raised in rabbits against a Ca binding protein extracted from rat skin, the cutaneous localization of this protein was studied by indirect immunofluorescence. The skin Ca binding immunoreactivity was found in the epidermis but not in the dermis; it was localized in the cytoplasm of the basal cell layer of both skin and Malpighian mucosa. There was no species specificity; this allowed the tracing of the protein in human epidermis as well where it was also expressed only in the basal cells. This is the 1st demonstration of the unique localization of a specific protein within the cytoplasm of the basal cell layer of the epidermis. This localization may help elucidate the physiological role of this protein.