FLUOROMETRIC ULTRAMICROANALYSIS OF DEOXYRIBONUCLEIC ACID IN HUMAN SKIN

[摘要] The deoxyribonucleic acid content of human epidermis and dermis has been determined by an ultramicromethod of fluorometric analysis. The procedure, described in detail, is a modification of the method described by Kissane and Robins for nerve tissue. It has been studied for application to morphologically defined fragments of human skin, namely epidermis, malpighian layer, horny layer, dermis, or homogenates of tissue. Since the method allows determinations of DNA on small (few micrograms) samples of tissue, it may be conveniently used in the study of biochemical variations in both normal and pathological conditions.