QUANTITATIVE HISTOCHEMISTRY OF PRIMATE SKIN .7. PYRUVATE KINASE

[摘要] Abundant pyruvate kinase activities were assayed in samples (averaging 1 [mu]) of various parts of the skin.The epidermis, except for the keratin and granular layers, the hair follicles and eccrine sweat glands contained from 10 to 15 moles/kg dry wt/hr. of pyruvate kinase activity. The sebaceous glands have less enzyme activities (4.5 to 6.5 moles/kg dry wt/hr.) than the other appendages. Epidermal pyruvate kinase activity is dependent on Mg++ and K+, and the latter ion is an absolute requirement for the enzyme. A possible physiological role of this enzyme, in addition to ATP (or energy) synthesis, might be that of a regulator in the glycolytic pathway by competing for a common substrate (ADP) with aerobic ATP synthesis via tricarboxylic add cycle.