ECCRINE SPIRADENOMA - HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDIES

[摘要] An electron microscopic and histochemical study of a case of eccrine spiradenoma with multiple lesions revealed: The tumor was dif?ferentiating towards eccrine rather than apocrine structures since it contained amylophosphorylase, branching enzyme and succinic dehydrogenase. When examined with an electron microscope the tumor was composed of small cells (basal cells) situated on or near the basement membrane, and many large cells (inner cells), some of which showed differentiation towards secretory cells. There were also glandular and tubular structures. The walls of the tubular lumina were composed of one layer of luminal cells and one or more layers of peripheral cells. The whole structure resembled the lower portion of the intradermal eccrine sweat duct, either adult or embryonic. The stroma between the tumor cords was composed of an amorphous hyaline substance which was produced by the basal cells, a fine fila?mentous substance and tropocollagen or precollagen fibrils produced by fibrocytes and peculiarly banded segments of mature collagen. Since these ultrastructural components showed positive histochemical reactions for PAS [periodic acidSchiff] stain (diastase-resistant) and Alcian blue and Hale stains (partially hyaluronidase labile), they contained neutral and acid mucopolysaccharides. The lumina of both glandular and tubular structures contained the same components as described in the stroma except for premature collagen and segments of mature collagen fibrils. The tubular lumina contained intact shafts and individual fibrils of the shafts of cilia. The basal bodies of these cilia were very often found in pairs in approximately 5% of the tumor cells of all types. The basal body ranged from 1,900 to 6,600 A in width and was composed of 7 to 9 peripheral fibrils measuring from 100-150 A in width. No central pair of fibrils were found. Both the basal body and the individual fibrils showed periodic cross-banding of approximately 640 A within which shorter and rather irregular banding of about 200-260 A was present.