STRATUM-CORNEUM STUDIES WITH PHOTOACOUSTIC SPECTROSCOPY

[摘要] Conventional spectroscopic studies on opaque membranes were difficult to pursue because of excessive light scattering and complications arising from the specimen''s surface. Recently, a new spectroscopic technique, photoacoustic spectroscopy, unlike conventional optical spectroscopy, was demonstrated to be an informative technique amenable to spectroscopic studies of solids and membrane-like samples. Photoacoustic spectroscopy was applied to the study of hydration and maturation of newborn rat stratum corneum, and clean spectra were obtained in the 220-450 nm region indicative of a change in thermal diffusivity with increased hydration, and biochemical changes associated with the initial maturation period.