PREPARATION AND CHARACTERIZATION OF A VIABLE SUSPENSION OF POSTEMBRYONIC HUMAN EPIDERMAL CELLS

[摘要] A method for preparing viable human epidermal cell suspensions is described. An epidermal cell suspension was prepared by tryptic separation of the epidermis followed by the mechanical removal of the epidermal cells from the whole separated epidermis. The cells were dissociated by further trypsinization to an essentially single cell suspension. Viability of the cells was demonstrated in culture by aggregation of cells, attachment to glass and outgrowth of cells forming monolayer sheets on glass. Epithelial cells and melanocytes were tentatively identified in culture. Total cell numbers and cell viability were measured.