IDIOPATHIC HYPERLIPEMIA AND PRIMARY HYPERCHOLESTEREMIC XANTHOMATOSIS .8. EFFECTS OF PROTAMINE ON THE ELECTROPHORETIC AND ULTRACENTRIFUGAL CHANGES PRODUCED IN THE SERUM BY HEPARIN

[摘要] Four patients with idiopathic hyperlipemia and 3 patients with primary hypercholesteremic xanthomatosis were given first an injection of heparin and then one of protamine. In idiopathic hyperlipemia, heparin produced in the electrophoretic pattern an increase in the speed of migration of the lipoproteins resulting in the development of a pre-albumin component representing the alpha lipoproteins. The subsequent administration of protamine caused disappearance of the pre-albumin component. In the ultracentrifugal pattern, heparin caused a shift of lipoproteins from the higher to the lower Sf classes. This shift was reversed by protamine. In primary hypercholesteremic xanthomatosis, heparin caused in the electrophoretic pattern an increase in the speed of migration of the beta lipoproteins so that they migrated with the speed of alpha-2 globulin. This acceleration in the speed of migration was abolished by protamine. Because the serum of our patients with primary hypercholesteremic xanthomatosis contained no lipoproteins of high Sf classes, heparin produced no changes in the ultracentrifugal pattern. Protamine produced no changes either. It is not possible to decide from our observations whether protamine acted by neutralizing heparin or by acting independently of heparin.