DNA MEASUREMENTS BY SINGLE NUCLEI FLOW-CYTOMETRY IN HUMAN ACTINIC SKIN-LESIONS

[摘要] A basal cell carcinoma, a Bowenoid carcinoma and clinically normal nonexposed skin from a 76-yr-old male with numerous epidermal tumors on sun-damaged skin were investigated by DNA flow cytometry, and the results were compared with data obtained from control group skin. From each type of skin lesion 3 different biopsies were examined. Each of the DNA frequency distribution histograms showed bimodal configuration revealing no more than 1 DNA stem line, which was diploid in the unaffected and Bowen carcinoma skin and hyperdiploid in the basal cell carcinoma. The 3 different types of skin could be clearly separated by means of at least 1 of 3 parameters: S-fraction, G2 + M fraction or SG2 + M index. The Sg2 + M index was the most sensitive discriminator for the tumors. The S-fraction level was higher in the clinically normal skin of the investigated male than in the control group and higher still in the tumors.