HISTOCHEMICAL OBSERVATIONS OF VERRUCA-VULGARIS

[摘要] Tissue sections of verruca vulgaris lesions were studied after staining with the fluorescent dye acridine orange. The nuclear inclusion bodies in the epidermal cells of the upper rete layer and the nuclei in the horny layer fluoresced yellow indicating the presence of DNA (deoxyribonucleic acid). A close correlation between results obtained with acridine orange and the Feulgen technique was noted. The DNA nuclear staining of cells infected with the verruca vulgaris virus was unchanged after the action of the enzyme DNAase, whereas the DNA of normal cells was readily degraded and failed to stain.