MAMMALIAN EPIDERMAL PROTEIN-SYNTHESIS - INITIATION-FACTORS

[摘要] There is a deficiency of initiation in protein-synthesizing systems prepared from mammalian epidermis. These systems do not respond to inhibitors of initiation although they remain sensitive to elongation inhibitors and exogenous ribonuclease. In spite of this deficiency of initiation, active protein factors which support initiation reactions are present in the KCl extract of mammalian epidermal ribosomes. A factor corresponding to the reticulocyte [initiation] factor IF-MP was isolated. An inhibitor of initiation is also present in the epidermal KCl wash. [Guinea pigs were used in these studies.].