SOME BIOCHEMICAL-PROPERTIES OF PEMPHIGOID ANTIGEN BOUND TO THE SURFACE OF DISSOCIATED EPIDERMAL BASAL CELLS

[摘要] Bullous pemphigoid antigen (BP Ag) is a cell surface marker of epidermal basal cells. The functional role of this molecule is unknown. Epidermal cell suspensions obtained by trypsinization of [rat] skin show a population of epidermal basal cells with a polar rim of antigen, as demonstrated by indirect immunofluorescence technique. Treatment of these cell suspensions with a variety of proteolytic and glycosidic enzymes failed to remove the antigen from these basal cells. BP Ag was stable upon incubation with distilled water, Triton X-100, PBS [phosphate buffered saline] and 1 M NaCl. Treatment of epidermal basal cells with 2 N NaSCN, 1% periodic acid and 4 M urea and acidic pH or 56.degree. C temperature, abolished the reactivity of these cells with BP antibodies.