A SIMPLE IN VIVO METHOD FOR STUDYING PERMEABILITY OF HUMAN STRATUM CORNEUM

[摘要] The permeability of the horny layer in vitro can be directly assessed by determining the time required for its complete permeation by the fluorescent dye tetra-chlorosalicylanilide. A 1.5 percent concentration in a solvent mixture of dimethylsulfoxide and ethylene glycol monomethyl ether is applied for varying lengths of time, the endpoint being the least time which enables dye penetration to the glistening layer as determined by stripping. The advantage is speed and simplicity; the results are useful for comparative purposes and are not influenced by such complicating factors as the density of follicles or the blood flow.