IMMUNOELECTRON MICROSCOPY OF TYPE-III COLLAGEN IN NORMAL AND SCLERODERMA SKIN

[摘要] Normal and scleroderma skin was studied by indirect immunoelectron microscopy with specific antibodies against Type III collagen. Fine collagen fibrils, 200-400 .ANG. in diameter, were labeled with antitype III collagen antibodies. These antibodies attached to the collagen fibril, forming rings with a 650 .ANG. periodicity. Fibrils with more than 600 .ANG. in diameter were only labeled with antitype I collagen antibodies. Type III collagen was found around small blood vessels, adipocytes and smooth muscle cells, corresponding to the distribution of reticulin.