PATTERNS OF GLUCOSE CATABOLISM DURING DIFFERENTIATION OF FETAL RAT EPIDERMIS

[摘要] Glucose metabolism was investigated during differentiation of fetal rat skin to evaluate shifting patterns in the catabolism of this major exogenous substrate in relation to changing requirements during epidermal maturation. Glucose utilization and aerobic glycolysis declined while oxidative metabolism increased in whole skin as stratification and cornification progressed. Activity of the hexose monophosphate shunt [HMP] was quantitated in isolated epidermis for the 1st time. The relative activity of this pathway diminished somewhat from 20 to 12% of utilized glucose as the granular layer and stratum corneum developed. Enhanced utilization of glucose, aerobic glycolysis and HMP activity in rapidly proliferating epidermis and flexibility of glucose catabolism in response to cellular requirements at different stages of differentiation was found.