EFFECTS OF CULTURAL CONDITIONS ON DEVELOPMENT OF ANTIGENS BY COCCIDIOIDES IMMITIS .2. COMPLEMENT FIXATION AND IMMUNODIFFUSION STUDIES WITH HUMAN SERUM

[摘要] The development of complement fixing antigen and antigen active immunodiffusion present in filtrates and ethylene glycol extracts of C. immitis cultured in a variety of media was studied, using a human antiserum. Satisfactory complement fixing antigen was obtained only from cultures in Sabouraud''s and brain heart infusion media. In all other media, complement fixing activity was either absent or completely masked by anticomplementary activity. Higher titers of complement fixing antigen were produced in cultures in brain heart infusion supplemented by the addition of dextrose compared to the unsupplemented medium. Anticomplementary activity could be removed by dialysis from cultures in brain heart infusion medium. No significant differences were found between the behavior in immunodiffusion of the human and rabbit antisera examined in this study. Antigens in culture filtrates from Roessler''s and asparagine media were detected in concentrations much lower than observed previously using the same antiserum, suggesting that deterioration of these antigens had occurred during storage.