ASSOCIATION OF LIPIDS WITH KERATINOUS PROTEINS OF HUMAN CALLUS

[摘要] Small amounts of phospholipids and cholesterol bound to major callus protein fractions during extraction were quantitated. Extraction with chloroform/methanol abolishes the requirement of these protein fractions for prolonged acid hydrolysis inorderto release completely certain amino acid residues. The binding capacity of lipid-free, isolated, keratinous proteins for cholesterol has been studied. A plausible (though unproven) role for phospholipids, cholesterol, and keratin in the barrier functions of epidermis is re-emphasized.