GLUTATHIONE-S-EPOXIDE TRANSFERASE IN MOUSE SKIN AND HUMAN FORESKIN

[摘要] In order to understand the molecular mechanisms involved in polycyclic hydrocarbon-induced skin tumorigenesis, analysis of the capability of skin to form and detoxify the oxide derivatives is of utmost importance. Glutathione-S-epoxide transferase activity in skin was determined in 6 mouse strains as well as in human foreskin (from 2-4-day-old neonates) using 2 substrates, styrene oxide and 3-methylcholanthrene-11,12-oxide. The highest enzyme activity was noted in the skin of the Balb/c mouse. The enzyme was not inducible in skin by prior administration of 3-methylcholanthrene to the mice. Human foreskin activity was lower than that present in the adult mouse skin and ranged from 1.93-5.28 with styrene oxide (at 22.degree. C) and 0.17-0.46 nmol/mg protein/5 min with 3-methylcholanthrene-11,12-oxide (at 37.degree. C) as substrates.