STRATUM CORNEUM FORMATION IN AUTO-IMPLANTS AND IN EXPLANTS OF HUMAN SKIN

[摘要] Proliferation and sites of stratum corneum formation were studied in auto-implants and in explants of split thicknesses of adult human skin. Keratin cysts, perles, and fistulae developed within epidermal cell nests and columns distal to the corium in implants. In explants, proliferation in Eagle''s media with added glutamine and serum was greater than in serum or Eagle''s media alone. Mitoses were seen only in Malpighian cells near the corium, even when the stratum corneum and epidermal barrier were removed to allow diffusion of the nutrient media into the epidermis from both surfaces. In such latter specimens keratohyaline granular cells and stratum corneum occurred most often in mid-epidermis, rather than in the site most distant from the corium. The hypothesis that stratum corneum formation depends on concentration gradients of diffusible substances is presented.