PREPARATION OF DERMATOPHYTES FOR AMINO ACID RESPIRATORY STUDIES

[摘要] L-amino-acid oxidase activity can be demonstrated in certain superficial dermatophytes after starvation while shaking in the presence of buffer. Endogenous amino-acid substrate separation in respiratory studies may be obtained, also, by the addition of L-alanine during shorter periods of starvation. A wide range of amino acids are utilized by the dermatophytes studied. Most of these organisms produced ammonia from a number of amino acids but with differing avidity. The ability to oxidize and to deaminate substrate differed according to the amino acid and with the organism. A strain of dermatophyte was found to undergo fragmentation without loss in physiological activity, thereby permitting accurate replication in quantitative metabolic studies. A definite correlation concerning utilization of ammonia and other products of deamination from various amino acids could not be obtained.