ON IMMUNOLOGICALLY-ACTIVE SUBSTANCES OF DERMATOPHYTES . I

[摘要] Twenty-two fractions having trichophytin activity were isolated from the mycelium and the culture filtrate of Tri-chophyton mentagrophytes var. asteroides cultured at 27[degree] C for 4 weeks on Sabouraud''s glucose broth. Any one of 4 crude fractions obtained from the mycelium according to a combination of Sakai'' s and Kono'' s methods was shown to be heterogeneous, giving rise to from 2 to 3 sub-fractions upon purification by the stepwise elution column chromato-graphy with DEAE-Sephadex A-50 and Tris-HCl system. Thus, 11 purified fractions having trichophytin activity were obtained from the mycelium. Three active fractions obtained from the culture filtrate by the ethanol fractional precipitation method were divided further into 11 sub-fractions by a similar chromatographic technic. The majority of these fractions was presumed to be polysaccharide substances containing a small quantity of protein or peptide. Some protein and nucleic acid fractions were also detected.