MEASUREMENT OF GLUTATHIONE IN HUMAN EPIDERMIS USING GLUTATHIONE REDUCTASE

[摘要] A quick, simple method for determining the oxidized and reduced glutathione content of tissue extracts or of serum samples is presented. Amounts of tissue in the 50-100 mg range are adequate. The smallest amount of glutathione which can be determined in the final reaction vessel is 2 X 10-U moles (equivalent of 20 micrograms of fresh tissue). Glutathione within the epidermis is over 90 percent reduced. The concentration of reduced glutathione ranges from 1.57 to 1.99 millimicromoles/mg of fresh tissue.