COMPARATIVE HISTOCHEMICAL OBSERVATIONS OF WOUND HEALING IN ADULT RATS AND CULTURED ADULT HUMAN EPITHELIUM .3. ALKALINE AND ACID PHOSPHATASE

[摘要] Histochemically detectable alkaline phosphatase during wound healing in rats was elevated at the time of eschar formation and later coincident with the laying down of collagen. Throughout this period, the epithelium which normally contains alkaline phosphatase in the granules of the stratum granulosum also revealed activity in the lower layers. This was distributed in the cytoplasm when stained by the Gomori or Seligman techniques following freeze-drying. After fixation in 80% alcohol the distribution was nuclear with the Gomori technique. Human epithelium contained no alkaline phosphatase in the outwandering cells during the period of culture. This was verified on both sectioned explants and whole mounts. The dermis also remained phosphatase free in culture. Acid phosphatase was only detected histochemically following freeze-drying. In normal skin it was limited to the granules of the stratum granulosum and the keratinized stratum corneum, none was found in the dermis. The migrating epithelium and newly formed dermis during wound healing was phosphatase free. In culture human skin explants contained acid phosphatase in the stratum granulosum and stratum corneum. The outwandering cells and dermis when examined in whole mounts or sections were phosphatase free.