SOLUBLE SUBSTANCES AND ANTIGENS OF HUMAN EPIDERMIS (ANALYSES BY ELECTROPHORESIS AND IMMUNOELECTROPHORESIS)

[摘要] Soluble substances extracted from human corporeal, palmar, plantar and mucosal epidermis and solutes extracted from hyperkeratosis callus and ichthyosis scales were studied by electrophoresis in starch gel and by immuno-electrophoresis. For the latter technic heterologous antisera against human plasma proteins and against human epidermis were used. Five substances of epidermal origin, with different antigenic determinants[long dash]immunologically unrelated to those of plasma proteins, were demonstrated in all extracts. The identity and nature of 2 substances, both of [beta]-globulins mobility, was determined by comparing their electrophoretic mobilities, their antigenic relationship and their chemical reactions with sulfhydryl and with carbohydrate reagents. The identity of 2 other types of substances[long dash]one of post albumin, the other of post [gamma] -globulin mobility[long dash]was deduced by noting their positions in the electrophoretic patterns and their immunological likeness; their nature was not determined. Both types were more abundant in cornified epidermis and in its horny derivatives.