THE EXPERIMENTAL PRODUCTION OF LEUKOCYTIC CHANGES IN NORMAL BLOOD SIMILAR TO LE-CELLS AND MORPHOLOGICALLY IDENTICAL WITH LE-CELLS

[摘要] Liquoid Roche, by direct coagulation-retardation, leads to phagocytosis of light to dark blue stained clumped thrombocytic masses, in the leukocytes of normal blood, resembling LE cells. Phagocytosis of decomposed nuclei and nucleoplasm is only of secondary importance. The Liquoid activity is independent of any serum factor. Various polymerization products of PVAS, through direct coagulation-retardation, also cause phagocytosis of decomposed and undecomposed nuclei and nucleoplasm. For all practical purposes the phagocytized material can be microscopically identified as to its origin. The PVAS effect is dependent on the gamma-globulin fraction of the serum, which perhaps combines with the substance, but is essentially changed. The leukocytic changes observed in this case are also very similar to the LE phenomemon, but not absolutely morphologically identical with them. The morphologically identical LE phenomenon changes (rosettes around amorphous acidophilic material and the phagocytosis of it) could only be produced with a serum that was treated with PVAS and heated. However the PVAS is a foreign substance to the body. It is suggested that the genuine LE phenomenon is based on the action of pathologic heparinoids in combination with the gamma-globulin fraction in the patients'' serum.